Figure 3

Production of vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1α (SDF-1α) by C2C12-Luc-GFP and C2C12-Luc-GFP-HO1 cells cultured in growth media (GM) or differentiation media (DM). (A) Expression of VEGF mRNA; quantitative RT-PCR (n = 3). (B) Secretion of VEGF protein to conditioned media; enzyme-linked immunosorbent assay (ELISA) (n = 5). (C) Expression of SDF-1α mRNA; quantitative RT-PCR (n = 3). (D) Secretion of SDF-1α protein to conditioned media; ELISA (n = 5). EF2 served as an internal control. Each bar represents mean + SEM; ^* p < 0.05 vs. C2C12-Luc-GFP cells. ^# P < 0.05, versus cells cultured in GM.