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Figure 5 | Stem Cell Research & Therapy

Figure 5

From: Myoblast-conditioned media improve regeneration and revascularization of ischemic muscles in diabetic mice

Figure 5

Histological and immunohistochemical analysis of ischemic muscles in hind limbs of db/db mice injected with conditioned media from C2C12-Luc-GFP, C2C12-Luc-GFP-HO1 cells or nonconditioned control media, performed on day 28 after injection of media (by quantitative analysis). (A) Angiogenesis – number of capillaries per microscopic field of view; immunohistochemical staining for CD31. (B) Arteriogenesis – number of arterioles per mm2; immunohistochemical staining for α-smooth muscle actin. (C) Myogenesis – percentage of regenerating fibers with centrally located nuclei; hematoxylin and eosin staining. Each point represents an individual animal. *P < 0.05, versus control; # P < 0.05, versus C2C12-Luc-GFP.

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