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Figure 10 | Stem Cell Research & Therapy

Figure 10

From: Systematic implantation of dedifferentiated fat cells ameliorated monoclonal antibody 1-22-3-induced glomerulonephritis by immunosuppression with increases in TNF-stimulated gene 6

Figure 10

Expression of TSG-6 in kidney from mAb 1-22-3-injected rats after implantation of DFAT cells and production of TSG-6 protein in cultured DFAT cells stimulated with TNF-α. Wistar rats were nephrectomized and injected without mAb 1-22-3 (Control) or with 0.5 mg mAb 1-22-3 through the tail vein seven days after nephrectomy. Thirty-five days after nephrectomy, saline (Saline) or 106 DFAT cells were injected through the renal artery (DFAT ia) or through the tail vein (DFAT iv) in rats. Sixty-three days after nephrectomy, all rats were killed and the left kidney was removed. Real-time quantitative PCR was performed for expression of TSG-6 (A) and TNF-α (B) mRNAs. C) Levels of TSG-6 in culture medium were determined using ELISA kits. D) Concentrations of TSG-6 in conditioned medium from 106 fibroblast or from 106 DFAT cells in culture were determined by ELISA kit. Serum levels of TSG-6 were determined in serum from Wistar rats injected with saline and 106 DFAT cells. Data are the mean ± SEM (n = 6). *P <0.05 and **P <0.01 in the indicated columns. DFAT, dedifferentiated fat; mAb, monoclonal antibody; SEM, standard error of the mean; TSG-6, TNF-stimulated gene-6.

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