Figure 11
Production of TSG-6 from mesangial cells (MCs) by transwell coculture of DFAT cells. MCs from stroke-prone spontaneously hypertensive rats (SHR-SP) were plated at 4,000 cells per cm2 in the bottom well of a six-well transwell coculture system. DFAT cells from Wistar rats were separately plated at 1,000 cells per cm2 on the upper inserts of a transwell coculture system and cultured for seven days. A) Transwell coculture systems of DFAT cells and MCs. MC: MCs alone. DFAT: DFAT cells alone. MC + DFAT: MCs were seeded in the lower chamber and DFAT cells were seeded in the upper chamber. B) Levels of TSG-6 in culture medium were determined using ELISA. C) Expression of TSG-6 mRNA was determined by real-time RT-PCR in MCs from SHR-SP or DFAT cells. Data are the mean ± SEM (n = 6). *P <0.05 and **P <0.01 in the indicated columns. DFAT, dedifferentiated fat; SEM, standard error of the mean; TSG-6, TNF-stimulated gene-6.