Figure 4

miR-21 regulates the reactive oxygen species level of dermal-derived cells. (A) Relative microRNA (miR)-21 levels of primary nonwounded dermal cells, granulation tissue-derived cells (GTCs), and GTCs pretreated with N-acetylcysteine (NAC; 5 mM) for 48 hours were tested by quantitative real-time PCR. (B) Relative reactive oxygen species (ROS) levels of primary nonwounded dermal cells, GTCs, and GTCs pretreated with miR-21 antagomir (50 nM) for 48 hours were detected by the ROS-sensitive dye 2′,7′-dichlorofluorescin diacetate (DCFH-DA; 10 mM). (C) Relative miR-21 levels of primary wild-type neonatal dermal cells, miR-21 knock-in neonatal dermal cells, and miR-21 knock-in neonatal dermal cells pretreated with NAC (5 mM) for 48 hours were detected by quantitative real-time PCR. (D) Relative ROS levels of primary wild-type neonatal dermal cells, miR-21 knock-in neonatal dermal cells, and miR-21 knock-in neonatal dermal cells pretreated with miR-21 antagomir (50 nM) for 48 hours were measured by DCFH-DA (10 mM). All results presented as mean ± standard deviation. d, days; DCF, 2′,7′-dichlorofluorescin; h, hours; miR-21 cells, miR-21 knock-in neonatal dermal cells; wild-type, wild-type neonatal dermal cells. **P < 0.01.