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Figure 2 | Stem Cell Research & Therapy

Figure 2

From: Lipopolysaccharide preconditioning of adipose-derived stem cells improves liver-regenerating activity of the secretome

Figure 2

Effects of conditioned medium (CM) and lipopolysaccharide (LPS)-CM on the viability of normal and hepatotoxic agent-treated mouse hepatocytes. (A) Untreated and 50 mM thioacetamide (TAA)-treated and TAA + TLR4 inhibitor (TAK-242) AML12 cells were incubated for 24 hours with the control medium, LPS (0.5 ng/mL), CM, and LPS-CM and cell viabilities were determined by using the Ez-Cytox cell viability assay. Values represent mean ± standard deviation of four independent experiments (P <0.05). (B) The expressions of phospho-signal transducer and activator of transcription 3 (p-STAT3) and STAT3 in AML12 cells treated as above were analyzed by Western blotting. *P <0.05. TLR4, Toll-like receptor 4.

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