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Figure 4 | Stem Cell Research & Therapy

Figure 4

From: Raman spectroscopy for grading of live osteosarcoma cells

Figure 4

Alizarin red S staining and von Kossa staining for mineralization of different osteosarcoma cells. (A) Alizarin red S staining represents the mineral distribution of human bone marrow-derived mesenchymal stem cells (MSCs), human fetal osteoblast (hFOB) cells, and osteosarcoma (MG63, SaOS2, and 143B) cells. Red spot, mineralization of five cells at 80% confluence under light microscope. Magnification: 20×. (B) Alizarin red S staining shows mineralization spots in the MG63 and hFOB group, but not in MSCs and SaOS2 and 143B cells. (C) Quantification of Alizarin red S stain by the absorbance (~570 nm) of the red extracts, normalized by the number of cells in the sample. *P <0.05, **P <0.01. (D) Von kossa staining shows phosphate mineralization spots in the MG63 and hFOB group but not in hMSCs and SaOS2 and 143B cells.

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