Skip to main content
Figure 6 | Stem Cell Research & Therapy

Figure 6

From: Hypoxic culture of bone marrow-derived mesenchymal stromal stem cells differentially enhances in vitro chondrogenesis within cell-seeded collagen and hyaluronic acid porous scaffolds

Figure 6

Immunohistochemical analysis of collagen II content within collagen and hyaluronic acid (HA) scaffolds seeded with hypoxia- and normoxia-cultured bone marrow-derived mesenchymal stem cells (BMSCs). BMSCs were isolated/expanded under normoxia or hypoxia, seeded within collagen or HA scaffolds, and subsequently differentiated under normoxia or hypoxia for 14 days in chondrogenic medium. Thereafter, constructs were sectioned at 5-μm thickness, and collagen II immunostaining was performed by using an aminoethylcarbazole-based peroxidase labeling kit. Photomicrographs represent collagen and HA scaffolds seeded with BMSCs from donor Z33.

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512