Table 2 Ovine primer sequences used in reverse transcription-quantitative real-time polymerase chain reaction analysis
Gene | Primer sequences | Â | NCBI reference |
|---|---|---|---|
β-actin (ACTB) | 5′-CGGCGGGACCACCAT-3′ | Forward | NM_001009784.1 |
5′-GCAGTGATCTCTTTCTGCATCCT-3′ | Reverse | ||
Aggrecan (ACAN) | 5′-TGGAATGATGTCCCATGCAA-3′ | Forward | XM_004018048.1 |
5′-GCCACTGTGCCCTTTTTACAG-3′ | Reverse | ||
COMP (COMP) | 5′-CCTAACTGGGTGGTGCTCAAC-3′ | Forward | XM_004009150.1 |
5′-CTGGGTCGCTGTTCATCGT-3′ | Reverse | ||
Collagen I (COL1A1) | 5′-CGCCCCAGACCAGGAATT-3′ | Forward | XM_004012773.1 |
5′-GTGGAAGGAGTTTACAGGAAGCA-3′ | Reverse | ||
Collagen II (COL2A1) | 5′-GACCTCACGTCTCCCCATCA-3′ | Forward | XM_004006408.1 |
5′-CTGCTCGGGCCCTCCTAT-3′ | Reverse | ||
Collagen X (COL10A1) | 5′-CAGGCTCGAATGGGCTGTAC-3′ | Forward | XM_004011185.1 |
5′-CCACCAAGAATCCTGAGAAAGAG-3′ | Reverse | ||
SRY-Box 9 (SOX9) | 5′-GCTGCTGGCCGTGATGA-3′ | Forward | XM_004013527.1 |
5′-GGGTCGCGCGTTTGTTT-3′ | Reverse |