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Table 2 Ovine primer sequences used in reverse transcription-quantitative real-time polymerase chain reaction analysis

From: Hypoxic culture of bone marrow-derived mesenchymal stromal stem cells differentially enhances in vitro chondrogenesis within cell-seeded collagen and hyaluronic acid porous scaffolds

Gene Primer sequences   NCBI reference
β-actin (ACTB) 5′-CGGCGGGACCACCAT-3′ Forward NM_001009784.1
5′-GCAGTGATCTCTTTCTGCATCCT-3′ Reverse
Aggrecan (ACAN) 5′-TGGAATGATGTCCCATGCAA-3′ Forward XM_004018048.1
5′-GCCACTGTGCCCTTTTTACAG-3′ Reverse
COMP (COMP) 5′-CCTAACTGGGTGGTGCTCAAC-3′ Forward XM_004009150.1
5′-CTGGGTCGCTGTTCATCGT-3′ Reverse
Collagen I (COL1A1) 5′-CGCCCCAGACCAGGAATT-3′ Forward XM_004012773.1
5′-GTGGAAGGAGTTTACAGGAAGCA-3′ Reverse
Collagen II (COL2A1) 5′-GACCTCACGTCTCCCCATCA-3′ Forward XM_004006408.1
5′-CTGCTCGGGCCCTCCTAT-3′ Reverse
Collagen X (COL10A1) 5′-CAGGCTCGAATGGGCTGTAC-3′ Forward XM_004011185.1
5′-CCACCAAGAATCCTGAGAAAGAG-3′ Reverse
SRY-Box 9 (SOX9) 5′-GCTGCTGGCCGTGATGA-3′ Forward XM_004013527.1
5′-GGGTCGCGCGTTTGTTT-3′ Reverse
  1. NCBI, National Center for Biotechnology Information.