Figure 3

Macrophage migration inhibitory factor induces rejuvenation in a concentration-dependent manner. (A) Proliferation growth curves of mesenchymal stem cells incubated with macrophage migration inhibitory factor (MIF) at concentrations of 1 to 1,000 ng/ml, determined by the Cell Counting Kit-8 (HaiGene Technology, Harbin, China) assay during 1, 3, 5 and 7 days of treatment. Each data point represents mean ± standard deviation from three independent experiments; *P <0.05 versus 100 ng/ml MIF. Relative concentration of (B) vascular endothelial growth factor (VEGF), (C) basic fibroblast growth factor (bFGF), (D) hepatocyte growth factor (HGF) and (E) insulin-like growth factor (IGF) analyzed by enzyme-linked immunosorbent assay. Each column represents mean ± standard deviation from three independent experiments; *P <0.05 versus 100 ng/ml MIF.