Figure 5From: Macrophage migration inhibitory factor confers resistance to senescence through CD74-dependent AMPK-FOXO3a signaling in mesenchymal stem cellsEffect of macrophage migration inhibitory factor on CD74 expression in mesenchymal stem cells. Expression of macrophage migration inhibitory factor (MIF) (A) mRNA analyzed by quantitative real-time PCR and (B) protein analyzed by western blot. Each column represents mean ± standard deviation from three independent experiments; P >0.05. (C) Densitometric quantification of MIF expression relative to internal control β-actin in young, aged and MIF-treated aged mesenchymal stem cells (MSCs). (D) Immunofluorescent staining of CD74 in young, aged and MIF-treated aged MSCs.Back to article page