Figure 6From: Macrophage migration inhibitory factor confers resistance to senescence through CD74-dependent AMPK-FOXO3a signaling in mesenchymal stem cellsMacrophage migration inhibitory factor function is mediated through CD74. (A) Western blot analysis of CD74 expression in untransfected mesenchymal stem cells (MSCs), and MSCs transfected with CD74-specific small interfering RNA (siRNA) and nontarget specific control scrambled small interfering RNA (siRNA-NT). (B) Densitometric quantification of CD74 expression relative to internal control β-actin in all three conditions. Each column represents mean ± standard deviation from three independent experiments; *P <0.05 versus siRNA-CD74. (C) Proliferation growth curves (determined by the Cell Counting Kit-8 (HaiGene Technology, Harbin, China) assay) of untransfected and untreated MSCs, and macrophage migration inhibitory factor (MIF)-treated control MSCs, CD74-siRNA transfected MSCs and siRNA-NT transfected MSCs. Each data point represents mean ± standard deviation from three independent experiments; *P <0.05 versus MIF; △ P <0.05 versus MIF + siRNA-NT. (D,E,F,G) Concentration of (D) vascular endothelial growth factor (VEGF), (E) basic fibroblast growth factor (bFGF), (F) hepatocyte growth factor (HGF) and (G) insulin-like growth factor (IGF) under normal and hypoxic conditions, in the culture medium of untransfected and untreated MSCs, and MIF-treated control MSCs, CD74-siRNA transfected MSCs and siRNA-NT transfected MSCs. Each column represents mean ± standard deviation from three independent experiments; *P <0.05 versus MIF; △ P <0.05 versus MIF + siRNA-NT.Back to article page