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Figure 6 | Stem Cell Research & Therapy

Figure 6

From: Macrophage migration inhibitory factor confers resistance to senescence through CD74-dependent AMPK-FOXO3a signaling in mesenchymal stem cells

Figure 6

Macrophage migration inhibitory factor function is mediated through CD74. (A) Western blot analysis of CD74 expression in untransfected mesenchymal stem cells (MSCs), and MSCs transfected with CD74-specific small interfering RNA (siRNA) and nontarget specific control scrambled small interfering RNA (siRNA-NT). (B) Densitometric quantification of CD74 expression relative to internal control β-actin in all three conditions. Each column represents mean ± standard deviation from three independent experiments; *P <0.05 versus siRNA-CD74. (C) Proliferation growth curves (determined by the Cell Counting Kit-8 (HaiGene Technology, Harbin, China) assay) of untransfected and untreated MSCs, and macrophage migration inhibitory factor (MIF)-treated control MSCs, CD74-siRNA transfected MSCs and siRNA-NT transfected MSCs. Each data point represents mean ± standard deviation from three independent experiments; *P <0.05 versus MIF; △ P <0.05 versus MIF + siRNA-NT. (D,E,F,G) Concentration of (D) vascular endothelial growth factor (VEGF), (E) basic fibroblast growth factor (bFGF), (F) hepatocyte growth factor (HGF) and (G) insulin-like growth factor (IGF) under normal and hypoxic conditions, in the culture medium of untransfected and untreated MSCs, and MIF-treated control MSCs, CD74-siRNA transfected MSCs and siRNA-NT transfected MSCs. Each column represents mean ± standard deviation from three independent experiments; *P <0.05 versus MIF; △ P <0.05 versus MIF + siRNA-NT.

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