hADSC tracing by histoimmunostaining with MAP2 antibody. MCAO mouse group with PBS injection instead of hADSC serving as the negative control (MCAO-Ctrl-PBS) (A), normal mice with blood vessel exposure only but not occlusion (MCAO-Sham) (B), MCAO mouse group with hADSC injection seven days after MCAO surgery (MCAO-hADSC) (C). Quantitative analysis of GFP labeled hADSCs, MAP2+ relative to the DAPI+ means neurons relative to the total cells in both the ipsilateral and contralateral areas of different groups of the MCAO brain, GFP relative to the DAPI+ means the survival hADSCs take the ratio of total brain cells, GFP+/MAP2+ relative to DAPI+ means the injected hADSCs which differentiated into MAP2+ cells take the ratio of total brain cells (D). *, ** and *** indicates that significant differences were found between groups (P<0.05, P<0.01 and P<0.001, respectively ). DAPI, 4',6-diamidino-2-phenylindole; hADSC, human adipose-derived stem cells; MAP2, microtubule-associated protein 2; MCAO, middle cerebral artery occlusion.