hADSC tracing by histoimmunostaining with GFAP antibody. MCAO mouse group with PBS injection instead of hADSC serving as the negative control (MCAO-Ctrl-PBS) (A), normal mice with blood vessel exposure only but not occlusion (MCAO-Sham) (B), MCAO mouse group with hADSC injection seven days after MCAO surgery (MCAO-hADSC) (C). Quantitative analysis of GFP labeled hADSCs, GFAP+ relative to the DAPI+ means astrocytes cells take the ratio of total cells in both the Ipsilateral and Contralateral of different groups of the MCAO brain, GFP relative to the DAPI+ means the survival hADSCs take the ratio of total brain cells, GFP + /GFAP + relative to DAPI + means the hADSCs transdifferentiated into astrocytes take the ratio of total brain cells (D). *, ** and *** indicates that significant differences were found between groups (P<0.05, P<0.01 and P<0.001, respectively). DAPI, 4',6-diamidino-2-phenylindole; GFAP, glial fibrillary acidic protein; hADSC, human adipose-derived stem cells; MCAO, middle cerebral artery occlusion.