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Figure 5 | Stem Cell Research & Therapy

Figure 5

From: Three-dimensional spheroid cell culture of umbilical cord tissue-derived mesenchymal stromal cells leads to enhanced paracrine induction of wound healing

Figure 5

UCX® spheroid-derived conditioned medium (CM3D) enhances immortalized keratinocyte cell line (HaCaT) and human dermal fibroblasts (HDF) cell migration, proliferation and elastin production. (A) Graphs represent the average area occupied by the HaCaT cells after 40 hours in contact with serum-free conditioned medium from three-dimensional cultures (CM3D), two-dimensional cultures (CM2D) and control (basal medium). Data are shown as mean ± standard deviation (SD); n = 9 to 12. (B) Representative images of HaCaT scratch-wound assays immediately after the scratches had been made (0 h) and then after 40 hours in the presence of serum-free CM3D, CM2D and control. (C) Graphs represent the average area occupied by the HDF after 40 hours with CM3D, CM2D and control. Data are shown as mean ± SD; n = 6 to 9. (D) Representative images of HDF scratch-wound assays immediately after the scratches had been made (0 h) and then after 40 hours in the presence of serum-free CM3D, CM2D and control. (E) HaCaT and HDF viability evaluated by methylthiazolyldiphenyl-tetrazolium bromide (MTT) reduction assay after 48 hours of incubation with CM2D and CM3D. Data are shown as percentage of control (Crtl) and as mean ± standard error of the mean; n = 3. (F) Elastin production by HaCaT and HDF at 24 and 72 hours post-incubation with CM3D, CM2D and control. Data are shown as mean ± SD; n = 2. *P < 0.05; **P < 0.01; *** P < 0.001. CM, conditioned medium.

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