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Figure 3 | Stem Cell Research & Therapy

Figure 3

From: Activation of Notch1 signalling promotes multi-lineage differentiation of c-KitPOS/NKX2.5POS bone marrow stem cells: implication in stem cell translational medicine

Figure 3

Functional identification of total and c-KitPOS/NKX2.5POS bone marrow mesenchymal stem cells. (A) Uninduced and induced bone marrow mesenchymal stem cells (BMSCs) and c-KitPOS/NKX2.5POS BMSCs were stained after 21 days of osteogenic or adipogenic differentiation with Alizarin Red and Oil Red O, respectively. (B) For smooth muscle cell differentiation, BMSCs were treated with transforming growth factor-β1-supplemented medium for 8 days, and then subjected to immunofluorescence (IF) staining for smooth muscle myosin heavy chain (SM-MHC). Target proteins were detected with fluorescein isothiocyanate-conjugated IgG. Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). Original magnification: (A) ×200. Scale bar: 30 μm.

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