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Figure 7 | Stem Cell Research & Therapy

Figure 7

From: Activation of Notch1 signalling promotes multi-lineage differentiation of c-KitPOS/NKX2.5POS bone marrow stem cells: implication in stem cell translational medicine

Figure 7

Jagged1 activation of Notch1 signalling induces multilineage differentiation of total and c-KitPOS/NKX2.5POS bone marrow mesenchymal stem cells. Total bone marrow mesenchymal stem cells (BMSCs) and c-KitPOS/NKX2.5POS BMSCs were classified into Jagged1, IgG, N-(2S-(3,5-difluorophenyl)acetyl)-l-alanyl-2-phenyl-1,1-dimethylethyl ester-glycine (DAPT), dimethyl sulfoxide (DMSO), and MOCK treatment groups (see Methods). After 8 days, the expression of differentiation markers for cardiomyocytes (NKX2.5 and cardiac troponin T (cTnT)), smooth muscle cells (SM22α), and endothelial cells (von Willebrand factor (vWF)) were examined by (A) real-time quantitative RT-PCR and (B) immunofluorescence staining. Triplicate experiments were performed for real-time quantitative RT-PCR analysis (n = 3). *P <0.05, **P <0.01, ## P <0.01, ### P <0.001 versus other associated groups. For immunofluorescence staining, target proteins were detected with phycoerythrin-conjugated or fluorescein isothiocyanate-conjugated IgG. Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). Representative images were captured by confocal microscopy. Scale bar: 50 μm.

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