Fig. 11

Ensheathing profiles issued by transplant-derived oligodendrocytes as shown by ultrastructural immunohistochemistry. Preparations are from an injured animal 3 month post-transplantation. a Companion toluidine blue-stained semi-thin section through the corpus callosum shows the co-localization of SC121(+) (brown) processes with blue myelin sheaths in transverse (arrow) axonal profiles. Co-localization profiles are dark brown. Asterisk shows a group of SC121(−) axons. b An SC121(+) process (arrowhead) is shown to ensheath an unlabeled axon. This profile is adjacent to one SC121(+) cell (1) and also one unlabeled cell (2). Cells 1 and 2 have the appearance of oligodendrocytes. c A magnification of the framed area in (b) shows detailed ultrastructural features of ensheathment by transplant-derived oligodendrocytes. SC121(+) tongue processes (arrowheads) are wrapped around a myelinated axon. Myelin sheath on the inside appears to be unlabeled. Scale bars: (a) = 5 μm; (b) = 1 μm; (c) = 500 nm