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Table 1 Primary antibodies used for immunocytochemistry, immunohistochemistry and ultrastructural immunohistochemistry
| Target phenotypes | Target proteins/epitopes | Host | Dilution | Vendor |
|---|---|---|---|---|
| Human cell markers | Human Nuclei Protein antibody (HNu) | Mouse | 1:1,000 | Millipore, Billerica, MA, USA |
| Human cytoplasm-specific antibody (SC121) | Mouse | 1:3,000 | StemCells, Inc., CA, USA | |
| Neuron (in vivo) | Type III-tubulin epitope J1 (TUJ1) | Rabbit | 1:400 | Covance, Berkeley, CA, USA |
| Neuron (in vitro) | Type III-tubulin epitope J1 (TUJ1) | Mouse | 1:100 | Sigma, Saint Louis, MO, USA |
| Mitotic marker | Ki67 antigen (NCL-Ki67p) | Rabbit | 1:400 | Novocastra Labs, Newcastle, UK |
| Axon | Neurofilament heavy chain | Rabbit | 1:200 | Sigma, Saint Louis, MO, USA |
| Progenitor/early oligodendrocyte | Olig2 | Goat | 1:50 | Santa Cruz, Santa Cruz, CA, USA |
| Platelet-derived growth factor receptor α (PDGFRα) | Rabbit | 1:100 | Santa Cruz, Santa Cruz, CA, USA | |
| A2B5 | Mouse | 1:200 | Millipore, Billerica, MA, USA | |
| NG2 | Rabbit | 1:100 | Millipore, Billerica, MA, USA | |
| O4 | Mouse | 1:100 | Millipore, Billerica, MA, USA | |
| Late oligodendrocyte | Myelin basic protein (MBP) | Rabbit | 1:200 | Abcam, Cambridge, MA, USA |
| Adenomatous polyposis coli protein (APC) | Rabbit | 1:200 | Novus Biologicals, Littleton, CO, USA | |
| Astrocyte (in vivo) | Glial fibrillary acidic protein (GFAP) | Rabbit | 1:400 | Dako, Carpinteria, CA, USA |
| Astrocyte (in vitro) | Glial fibrillary acidic protein (GFAP) | Rabbit | 1:100 | Millipore, Billerica, MA, USA |
| Mesodermal marker | Bone morphogenetic protein-4 (BMP4) | Mouse | 1:300 | Millipore, Billerica, MA, USA |
