|
Human cell markers
|
Human Nuclei Protein antibody (HNu)
|
Mouse
|
1:1,000
|
Millipore, Billerica, MA, USA
|
| |
Human cytoplasm-specific antibody (SC121)
|
Mouse
|
1:3,000
|
StemCells, Inc., CA, USA
|
|
Neuron (in vivo)
|
Type III-tubulin epitope J1 (TUJ1)
|
Rabbit
|
1:400
|
Covance, Berkeley, CA, USA
|
|
Neuron (in vitro)
|
Type III-tubulin epitope J1 (TUJ1)
|
Mouse
|
1:100
|
Sigma, Saint Louis, MO, USA
|
|
Mitotic marker
|
Ki67 antigen (NCL-Ki67p)
|
Rabbit
|
1:400
|
Novocastra Labs, Newcastle, UK
|
|
Axon
|
Neurofilament heavy chain
|
Rabbit
|
1:200
|
Sigma, Saint Louis, MO, USA
|
|
Progenitor/early oligodendrocyte
|
Olig2
|
Goat
|
1:50
|
Santa Cruz, Santa Cruz, CA, USA
|
|
Platelet-derived growth factor receptor α (PDGFRα)
|
Rabbit
|
1:100
|
Santa Cruz, Santa Cruz, CA, USA
|
| |
A2B5
|
Mouse
|
1:200
|
Millipore, Billerica, MA, USA
|
|
NG2
|
Rabbit
|
1:100
|
Millipore, Billerica, MA, USA
|
| |
O4
|
Mouse
|
1:100
|
Millipore, Billerica, MA, USA
|
|
Late oligodendrocyte
|
Myelin basic protein (MBP)
|
Rabbit
|
1:200
|
Abcam, Cambridge, MA, USA
|
| |
Adenomatous polyposis coli protein (APC)
|
Rabbit
|
1:200
|
Novus Biologicals, Littleton, CO, USA
|
|
Astrocyte (in vivo)
|
Glial fibrillary acidic protein (GFAP)
|
Rabbit
|
1:400
|
Dako, Carpinteria, CA, USA
|
|
Astrocyte (in vitro)
|
Glial fibrillary acidic protein (GFAP)
|
Rabbit
|
1:100
|
Millipore, Billerica, MA, USA
|
|
Mesodermal marker
|
Bone morphogenetic protein-4 (BMP4)
|
Mouse
|
1:300
|
Millipore, Billerica, MA, USA
|
