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Table 1 Primary antibodies used for immunocytochemistry, immunohistochemistry and ultrastructural immunohistochemistry

From: Transplantation of human oligodendrocyte progenitor cells in an animal model of diffuse traumatic axonal injury: survival and differentiation

Target phenotypes Target proteins/epitopes Host Dilution Vendor
Human cell markers Human Nuclei Protein antibody (HNu) Mouse 1:1,000 Millipore, Billerica, MA, USA
  Human cytoplasm-specific antibody (SC121) Mouse 1:3,000 StemCells, Inc., CA, USA
Neuron (in vivo) Type III-tubulin epitope J1 (TUJ1) Rabbit 1:400 Covance, Berkeley, CA, USA
Neuron (in vitro) Type III-tubulin epitope J1 (TUJ1) Mouse 1:100 Sigma, Saint Louis, MO, USA
Mitotic marker Ki67 antigen (NCL-Ki67p) Rabbit 1:400 Novocastra Labs, Newcastle, UK
Axon Neurofilament heavy chain Rabbit 1:200 Sigma, Saint Louis, MO, USA
Progenitor/early oligodendrocyte Olig2 Goat 1:50 Santa Cruz, Santa Cruz, CA, USA
Platelet-derived growth factor receptor α (PDGFRα) Rabbit 1:100 Santa Cruz, Santa Cruz, CA, USA
  A2B5 Mouse 1:200 Millipore, Billerica, MA, USA
NG2 Rabbit 1:100 Millipore, Billerica, MA, USA
  O4 Mouse 1:100 Millipore, Billerica, MA, USA
Late oligodendrocyte Myelin basic protein (MBP) Rabbit 1:200 Abcam, Cambridge, MA, USA
  Adenomatous polyposis coli protein (APC) Rabbit 1:200 Novus Biologicals, Littleton, CO, USA
Astrocyte (in vivo) Glial fibrillary acidic protein (GFAP) Rabbit 1:400 Dako, Carpinteria, CA, USA
Astrocyte (in vitro) Glial fibrillary acidic protein (GFAP) Rabbit 1:100 Millipore, Billerica, MA, USA
Mesodermal marker Bone morphogenetic protein-4 (BMP4) Mouse 1:300 Millipore, Billerica, MA, USA