Table 2 Limiting factors for improving the decellularization/recellularization technology
From: Current achievements and future perspectives in whole-organ bioengineering
Topic of interest | Primary issue | Research goals |
|---|---|---|
Protocols of decellularization | Standardization of decellularization methods for each organ | Achievement of a reproducible method to obtain scaffold from different organs and different species |
Cell source | Identification of suitable cell source | Recellularization of whole-organ scaffolds |
Autologous cells | ||
Heterologous cells | ||
Large animal model | Creation of models for transplantation with a long-term functional follow-up | Obtain organs for human clinical transplantation |