Fig. 1

High density lipoprotein promotes proliferation of adipose-derived stem cells. a Characterization of mice adipose-derived stem cell (ADSC) markers: cells were labeled with fluorescein isothiocyanate (FITC)-conjugated and phycoerythrin (PE)-conjugated antibodies and evaluated by flow cytometry. After three passages, ADSCs were positive for CD44 and Sca-1, but negative for CD34 and CD45. Black lines, IgG isotype control. b MTT assay and d bromodeoxyuridine (BrdU) assay: cell proliferation in mice ADSCs treated with different concentrations (0, 20, 50, 100, 200 μg/ml) of high density lipoprotein (HDL) for 24 hours. c MTT assay and e BrdU assay: cell proliferation of mice ADSCs treated with HDL over various time periods (6 to 48 hours). f MTT assay and h BrdU assay: cell proliferation in human ADSCs treated with different concentrations (0, 20, 50, 100, 200 μg/ml) of HDL for 24 hours. g MTT assay and i BrdU assay: cell proliferation of human ADSCs over various time periods (6 to 48 hours). Bar graphs represent mean ± standard error of the mean, ***P <0.001. PBS, phosphate-buffered saline