Table 1 Primers used in quantitative reverse transcription-polymerase chain reaction
Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
Wnt3a | CATCGCCAGTCACATGCACCT | CGTCTATGCCATGCGAGCTCA |
Wnt5a | GCCACTTGTATCAGGACCACA | GGCATTTACCACTCCAGCAG |
Wnt11 | TACCTGCTTGACCTGGAGAG | TAGGAGCATCGGAAAACTTG |
Fzd3 | GTGTGTTTTGTCGGCCTCTACG | GAATGTGATACTCTCTGCAGCGTTC |
β-catenin | CTGACCAAACTGCTAAATGACG | GATGGTGGGAAAGGTTGTGTAG |
GSK-3β | TCGCCACTCGAGTAGAAGAAA | ACTTTGTGACTCAGGAGAACT |
C-myc | TCTTGGAACGTCAGAGGAGAA | GCTTGAACGGACAGGATGTAG |
Tcf4 | GCCTCTCATCACGTACAGCA | GGATGGGGGATTTGTCCTAC |
APC | TGGAATGGTGAGTGGGATTG | GGGTCTTGCTTCTGCTTGGT |
Cyclin D | AGGCGGATGAGAACAAGCA | GAGGGTGGGTTGGAAATGAA |
Atoh1 | CCAGGGTGAGCTGGTAAGG | CGTTGTTGAAGGACGGGAT |
Olfm4 | GCCAGCACTGGTAACATA | GCGAGTAGCGAAAGAAC |
β-actin | GTAAAGACCTCTATGCCAACA | GGACTCATCGTACTCCTGCT |