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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Influence of erythropoietin on microvesicles derived from mesenchymal stem cells protecting renal function of chronic kidney disease

Fig. 2

Analysis of the expression of surface markers and characterization of MSCs and EPO-MSC. a MSCs and EPO-MSC were labeled with the antibody against CD45, CD105, CD90 and CD44, the cells present were positive for CD105, CD90 and CD44 but negative for CD45. b Representative FACS analyses of MSC-MVs and EPO-MVs expressed similar results in CD44, CD29 and α4-integrin. c As described in Methods, MSC and EPO incubation MSC(EPO-MSC) undifferentiated, when 50 to 60 or 70 to 80 % confluent, were cultured in conditions that were inducive of osteogenic or adipogenic differentiation, respectively. After osteogenic differentiation, calcium in the mineralized extracellular matrix was shown by Alizarin Red S staining. After adipogenic differentiation, lipid droplets were indicated by their staining with Oil Red O. EPO erythropoietin FACS fluorescence-activated cell sorting MSC mesenchymal stem cells

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Stem Cell Research & Therapy

ISSN: 1757-6512