Fig. 4

The expression of E-cadherin and α-SMA in HK2 cells. a The analysis of gene expression of E-cadherin and a-SMA in HK2 cells following TGF-β1 (6 ng/ml) by RT-PCR at two time points (48 and 72 h) with MVs or EPO-MVs. All of the experiments were repeated three times (n = 3). b Western blot was used to detect the protein expression of E-cadherin and α-SMA in HK2 cells following TGF-β1 (6 ng/ml) at two time points (48 and 72 h) with MVs or EPO-MVs. All of the experiments were repeated three times (n = 3).*p < 0.01, 48-h TGF-β1 group versus HK-2 group or 72-h TGF-β1 group versus HK-2 group, ^$ p < 0.01, 48-h HK2 + TGF-β1 + MV group versus TGF-β1 group or 72-h HK2 + TGF-β1 + MV group versus TGF-β1 group, ^# p < 0.05, 48-h HK2 + TGF-β1 + EPO-MV group versus HK2 + TGF-β1 + MV group or 72-h HK2 + TGF-β1 + EPO-MV group versus HK2 + TGF-β1 + MV. EPO erythropoietin HK2 human kidney 2 MVs microvesicles TGF-β1 transforming growth factor-β1 α-SMA α-smooth muscle actin