Fig. 6

Effect of intravenous injection of MVs or EPO-MVs into unilateral ureteral obstruction (UUO) mice. a Mice were subjected to unilateral ureteral obstruction on day 0, followed by intravenous injection of MVs or EPO-MV on day 1 (n = 5 each group). Creatinine and blood urea nitrogen values were determined at the beginning of the experiments and on days 7 and 14 after surgery. *p < 0.01, day 7 UUO group versus control group or day 14 UUO group versus control group, ^$ p < 0.01, day 7 UUO + MV group versus UUO group or day 14 UUO + MV group versus UUO group, ^# p < 0.05, day 7 UUO + EPO-MV group versus UUO + MV group or day 14 UUO + EPO-MV group versus UUO + MV group. b Representative micrographs of renal histology of the kidney sections with hematoxylin and eosin (HE) and Masson’s trichrome (MT) at days 7 and 14 after surgery in control mice, in UUO mice injected with 30 μg of MVs or EPO-MV. Magnification: ×200. c Immunohistochemistry of α-SMA and E-cadherin in renal histology of the kidney sections at days 7 and 14 after surgery in control mice and in UUO mice with or without 30 μg MVs or EPO-MV. EPO erythropoietin MVs microvesicles UUO unilateral ureteral obstruction α-SMA α-smooth muscle actin