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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Donor mesenchymal stem cell-derived neural-like cells transdifferentiate into myelin-forming cells and promote axon regeneration in rat spinal cord transection

Fig. 1

Identification of TrkC or NT-3 gene transfection and neural differentiation of co-cultured TrkC-MSCs and NT-3-MSCs in the 3D GS scaffold. a, b MSCs at 2 days after infected with Ad-TrkC and Ad-NT-3 in vitro, respectively. Note that TrkC-positive (a) and NT-3-positive (b) MSCs are evident. c, d Bar charts show the percentage of Tju-1-positive (c) and Map2-positive (d) cells in the M, MN, MT, and MN + MT groups. Asterisk indicates statistical significance compared with the M group (*P < 0.05), pound sign indicates significance compared with the MN group (# P < 0.05), and ampersand indicates significance compared with the MT group (& P < 0.05). The data were presented as mean ± standard deviation (n = 5), and one-way analysis of variance with least significant difference test statistics was performed. e Cells in the 3D GS scaffold were immunostained by Tju-1 and Map2 antibodies 14 days after culture. Tju-1-positive and Map2-positive cells were observed in all groups. Scale bars = 20 μm. 3D, Three-dimensional; GS, Gelatin sponge; M, MSCs; Map2, Microtubule-associated protein 2; MN, NT-3-MSCs; MSCs, Mesenchymal stem cells; MT, TrkC-MSCs; NT-3, Neurotrophin-3; Tju-1, β-tubulin III; TrkC, Tropomyosin receptor kinase C

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