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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Donor mesenchymal stem cell-derived neural-like cells transdifferentiate into myelin-forming cells and promote axon regeneration in rat spinal cord transection

Fig. 4

Myelin sheath formation in the injury/graft site of spinal cord at 8 weeks after mesenchymal stem cell (MSC)-derived neural-like cell transplantation. ad Co-localization of green fluorescent protein (GFP) and myelin basic protein (MBP) in an MSC-derived neural-like cell as observed under a confocal microscope. The ortho section showed that the grafted GFP-positive cell was immunostained by MBP antibody and encircled the host neurofilament 200 (NF200)-positive axon (white arrowheads). e, f A myelin sheath was formed by a GFP-positive cell under the GFP-immunoelectron microscope observation. The red box in (e) was magnified in (f). f Red arrows indicated GFP-positive electron-dense 3, 3′-diaminobenzidine (DAB) deposited on the myelin sheath, and red arrowheads indicated GFP-positive electron-dense DAB deposited on the surface of nucleus. Asterisk indicates the nucleus of MSC-derived myelin-forming cell (f). g A GFP-positive cell deposited with DAB and counterstained by toluidine blue formed myelin-like structure (red arrowheads) in semithin section. Asterisk indicates DAB-positive cell body (g). Scale bars = 20 μm (a–d)

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