Fig. 4
From: Wnt and Notch signaling pathway involved in wound healing by targeting c-Myc and Hes1 separately
The relationships of the Wnt and Notch signaling pathway and the proliferation of epidermal stem cells was analyzed by immunofluorescence. a and c. Representative BrdU/c-Myc double-positive cells in wounded skin on day 7 (a), and the percentage of the positive cells to total cells in wound tissue at the indicated post-wounding time points (c). b and d. Representative BrdU/Hes1 double-positive cells in wounded skin on day 7 (b), and the percentage of the positive cells to total cells in wound tissue at the indicated post-wounding time points (d). *P < 0.01, **P < 0.05 compared with the control value (n = 5). Original magnification, 100×. Scale bar = 100 μm. BrdU 5-bromodeoxyuridine, DAPT N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester, DKK1 Dickkopf-1, Hes hairy and enhancer of split, LiCl lithium chloride, rhNF-κB recombinant human nuclear factor-kappa-B