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Table 1 Expression of cell surface antigens and secreted proteins in BS cells and BMSCs

From: Characterization of bursa subacromialis-derived mesenchymal stem cells

Antigen

Manufacturer

Label

Marker specification

Positive cells (%)

BS cells

BMSC

CD34

Beckman Coulter

PE

Hematopoietic stem cell marker, cell adhesion

7.69 (+)

1.05 (–)

CD53

AbD Serotec

FITC

Osteoblast and osteoclast signal transduction

0.00 (–)

0.14 (–)

CD73

BD Biosciences

PE

Mesenchymal, epithelial and endothelial cell marker

97.46 (+++)

98.67 (+++)

CD90

Beckman Coulter

PE

Fibroblast, stromal and hematopoietic stem cell marker

95.18 (+++)

98.37 (+++)

CD105

Beckman Coulter

PE

Mesenchymal and erythroid progenitor cells

98.05 (+++)

99.10 (+++)

CD106

AbD Serotec

FITC

Cell adhesion

4.09 (–)

55.55 (++)

CD133

Beckman Coulter

PE

Hematopoietic stem cell marker

0.14 (–)

1.20 (–)

CD144

AbD Serotec

FITC

Endothelial cells, cell adhesion

0.01 (–)

0.58 (–)

CD166

BD Biosciences

PE

Mesenchymal, epithelial stem cells, fibroblasts, monocytes, cell adhesion

88.06 (++)

98.42 (+++)

ALP

R&D Systems

APC

Alkaline phosphatase

22.79 (+)

55.50 (++)

FGF

R&D Systems

FITC

Fibroblast growth factor

45.06 (+)

42.64 (+)

Stro1

Dako

FITC

Mesenchymal stem cell marker

0.15 (–)

0.68 (–)

  1. +++ marker expression on >95 % of the cells, ++ marker expression on 50-95 % of the cells, + marker expression on 5-50 % of the cells, - marker expression on <5 % of the cells
  2. ALP alkaline phosphatase, APC allophycocyanin, BMSC bone marrow-derived MSC, BS bursa subacromialis, CD cluster of differentiation, FGF fibroblast growth factor, FITC fluorescein isothiocyanate, MSC mesenchymal stem cells, PE phycoerythrin