Fig. 3
Increased number of conjunctive and osteogenic progenitor cells in concentrated BMMC. a Scatter plots showing the distribution of total mononuclear cell number in 1 × 106 cells/mL after bone marrow aspiration (BMA) and after concentration of bone marrow-derived mononuclear cells (BMMC) on Ficoll density gradient centrifugation. b, c Flask and morphologic characteristics of colony-forming unit-fibroblasts (CFU-F) from BMMC. d, e Flask with mineralized matrix stained with red Alizarin-S and Crystal Violet of colony-forming unit-osteoblasts (CFU-O) from BMMC. f Frequency of CFU-F/106 cells and CFU-O/106 cells in concentrated BMMC. g Frequency of CFU-F/106 cells isolated in BMMC from individuals with osteonecrosis at a distant site was independent of the disease stage. The high standard deviation indicates the high variability of CFU-F and CFU-O within the SCD bone marrow. Data are represented as individuals values. Horizontal bars indicate mean value. Scale bar: 50 μm. ***P < 0.001. NS, not significant