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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: iTRAQ-based analysis of progerin expression reveals mitochondrial dysfunction, reactive oxygen species accumulation and altered proteostasis

Fig. 3

Immunoblotting and RT-PCR verification of selected proteins. a Western blotting analysis confirms the modulation of Lamin A, calnexin (CALX), nucleolin (NCL), and cytochrome c (CYC). Loading of equal amount of total protein was tested by Ponceau red staining (not shown) and α-tubulin immunoblotting. b Real-time PCR analysis of the selected proteins normalized against Hprt gene levels. *Significance (p <0.05) using the Kruskal–Wallis non-parametric test. **Significance (p <0.01) using the Kruskal–Wallis non-parametric test. iTRAQ isobaric tags for relative quantification, PG progerin

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Stem Cell Research & Therapy

ISSN: 1757-6512