Skip to main content

Table 1 Modulated proteins (p <0.05) in PG-3T3L1 cells classified according to their principal biological process

From: iTRAQ-based analysis of progerin expression reveals mitochondrial dysfunction, reactive oxygen species accumulation and altered proteostasis

Accession number

Name

Total number of peptides

iTRAQ 1 ratio PG/control

iTRAQ 2 ratio PG/control

Actin cytoskeleton organization

 gi|157951604

Adenylyl cyclase-associated protein 1

9

1.80

1.81

 gi|6680924

cofilin-1

22

2.63

2.63

 gi|6681069

Cysteine and glycine-rich protein 1

9

0.41

0.44

 gi|62526118

Cytoskeleton-associated protein 4

22

6.02

5.81

 gi|125347376

Filamin-A

123

0.06

0.06

 gi|145966915

Filamin-B

89

0.18

0.22

 gi|124487139

Filamin-C

51

0.42

0.44

 gi|130488506

Four and a half LIM domains protein 3

8

0.39

0.34

 gi|61657921

Kinesin-1 heavy chain

14

0.49

0.44

 gi|6755040

Profilin-1

19

2.90

2.94

 gi|10946578

Thymosin beta-4

15

0.42

0.44

 gi|47894398

Tropomyosin alpha-4 chain

51

0.57

0.60

 gi|31982755

Vimentin

101

27.8

29.5

Alternative splicing

 gi|85060507

Heterogeneous nuclear ribonucleoprotein A1

27

6.94

8.24

 gi|21313308

Heterogeneous nuclear ribonucleoprotein M

17

2.70

2.85

 gi|283436178

Heterogeneous nuclear ribonucleoproteins C1/C2

10

1.82

1.85

 gi|6678143

Lupus La protein homolog

9

1.57

1.89

 gi|31560656

Polyadenylate-binding protein 1

24

1.55

1.88

 gi|126157504

Serine/arginine repetitive matrix protein 2

12

0.33

0.35

 gi|34328400

Serine/arginine-rich splicing factor 1 isoform 1

12

0.58

0.63

 gi|153791358

Splicing factor 3B subunit 1

7

0.50

0.52

 gi|268837785

Splicing factor 3b, subunit 2

16

1.59

1.62

 gi|23956214

Splicing factor, proline- and glutamine-rich

22

1.48

1.45

ATP metabolism

 gi|6680748

ATP synthase subunit alpha

21

2.53

2.48

 gi|31980648

ATP synthase subunit beta

32

5.89

5,84

 gi|7949005

ATP synthase-coupling factor 6

8

0.35

0.38

 gi|150456419

ATP-dependent RNA helicase A

10

0.52

0.45

 gi|134288917

Cytoplasmic dynein 1 heavy chain 1

16

0.48

0.53

Calcium-mediated signalling

 gi|6996913

Annexin A2

28

1.89

1.72

 gi|6753060

Annexin A5

26

6.01

6.47

 gi|21704156

Caldesmon 1

31

2.17

2.49

 gi|6753244

Calmodulin

22

0.31

0.31

 gi|6671664

Calnexin

14

1.62

2.44

 gi|21312564

Calponin-3

15

2.01

1.79

 gi|6680836

Calreticulin precursor

31

3.37

3,98

 gi|31981086

EF-hand domain-containing protein D2

6

0.39

0.40

 gi|6679465

Glucosidase 2 subunit beta precursor

9

0.59

0.69

 gi|33620739

Myosin light polypeptide 6

24

0.69

0.39

 gi|6677833

Protein S100-A10

11

0.28

0.31

 gi|6677691

Reticulocalbin-1 precursor

10

0.50

0.69

 gi|114205428

Reticulocalbin-2 precursor

12

0.48

0.45

 gi|188035858

Reticulocalbin-3 precursor

13

0.41

0.54

 gi|295054266

Spectrin alpha chain, brain isoform 2

80

0.60

0.58

 gi|117938332

Spectrin beta chain, brain 1 isoform 1

45

0.50

0.43

Cell adhesion

 gi|61743961

AHNAK nucleoprotein isoform 1

265

0.63

0.62

 gi|6754508

LIM and SH3 domain protein 1

15

0.55

0.59

 gi|225543161

Lipoma-preferred partner homolog isoform 1

18

0.55

0.55

 gi|70778915

Moesin

41

3.31

2.80

 gi|33598964

Myosin-10

42

0.18

0.19

 gi|114326446

Myosin-9 isoform 1

127

0.05

0.04

 gi|254675244

Plectin isoform 1

151

0.31

0.56

 gi|377835925

Protein AHNAK2-like

16

0.78

0.54

 gi|227116327

Talin-1

42

0.25

0.24

 gi|6678347

Thy-1 membrane glycoprotein preproprotein

5

4.15

3.93

 gi|6756085

Zyxin

19

0.52

0.55

 gi|114326497

Laminin subunit beta-1

5

0.34

0.37

 gi|31982030

Rho GDP-dissociation inhibitor 1

10

2.60

2.49

DNA replication

 gi|6679299

Prohibitin

9

1.82

2.39

 gi|7242171

Proliferating cell nuclear antigen

10

1.73

1.78

Extracellular matrix organization

 gi|34328108

Collagen alpha-1(I) chain precursor

56

0.66

0.49

 gi|111120329

Collagen alpha-2(I) chain precursor

53

0.67

0.68

 gi|33859580

Galectin-3

12

1.96

1.81

 gi|33859596

Prolyl 4-hydroxylase subunit alpha-1 precursor

14

1.89

1.89

Glycolysis

 gi|6679937

Glyceraldehyde-3-phosphate dehydrogenase

33

25.5

23.1

 gi|70778976

Phosphoglycerate kinase 1

40

3.37

3.18

 gi|226958349

Triosephosphate isomerase

20

2.58

2.53

 gi|70794816

Alpha enolase

55

16.8

20.2

Golgi apparatus function

 gi|254750698

Nucleobindin-1 isoform 1 precursor

17

0.53

0.54

 gi|194440700

Nucleobindin-2 isoform 1 precursor

5

0.53

0.54

Lipid metabolism

 gi|6681137

Acyl-CoA-binding protein isoform 2

10

0.29

0.29

 gi|63999380

Alpha-2-macroglobulin receptor-associated

4

0.40

0.42

 gi|313151222

ATP-citrate synthase isoform 1

10

0.46

0.53

 gi|93102409

Fatty acid synthase

8

0.55

0.55

 gi|225735657

Sulphated glycoprotein 1 isoform F preproprotein

9

5.49

5.24

Lipid transport

 gi|19527028

Vigilin

15

1.52

1.47

 gi|38198665

NSFL1 cofactor p47

13

0.69

0.64

Nuclear structure

 gi|162287370

Prelamin-A/C isoform A

47

4.62

4.79

 gi|7110705

Prothymosin alpha

7

0.42

0.43

Nucleosome assembly

 gi|7949045

Histone H2A.Z

6

1.73

1.49

 gi|13591862

Protein SET isoform 1

21

0.22

0.27

Nucleotide biosynthesis

 gi|209862992

Dihydropyrimidinase-related protein 3 isoform 1

18

1.95

1.75

 gi|377835587

inosine-5′-monophosphate dehydrogenase 2-like

9

1.47

1.68

Oxidation–reduction process

 gi|31542438

Cytochrome b5 type B precursor

6

3.05

3.20

 gi|112181182

Cytochrome c oxidase subunit 5A

12

0.35

0.28

 gi|6681095

Cytochrome c, somatic

9

0.44

0.41

 gi|112293264

Protein disulphide-isomerase A3 precursor

49

6.03

5.80

 gi|86198316

Protein disulphide-isomerase A4 precursor

27

1.55

1.61

 gi|42415475

Protein disulphide-isomerase precursor

44

1.86

1.87

 gi|6755911

Thioredoxin

10

0.47

0.47

Protein catabolic process

 gi|6679501

26S protease regulatory subunit 4

15

2.21

2.59

 gi|228008337

26S protease regulatory subunit 6A

16

1.97

1.93

 gi|124248577

26S protease regulatory subunit 6B

12

2.27

2.37

 gi|19882201

26S proteasome non-ATPase regulatory subunit

9

0.54

0.52

 gi|6755212

Proteasome activator complex subunit 1

12

0.60

0.58

Protein folding and stress response

 gi|6680309

10 kDa heat shock protein, mitochondrial

8

0.34

0.34

 gi|183396771

60 kDa heat shock protein, mitochondrial

46

23.9

13.9

 gi|115270960

BAG family molecular chaperone regulator 3

9

0.57

0.59

 gi|6755863

Endoplasmin precursor

42

6.42

7.51

 gi|31981690

Heat shock cognate 71 kDa protein

68

14.4

19.9

 gi|6754254

Heat shock protein HSP 90-alpha

44

2.22

2.26

 gi|40556608

Heat shock protein HSP 90-beta

48

2.55

2.46

 gi|27229055

Huntingtin-interacting protein K

5

0.40

0.42

 gi|161353506

Serpin H1 precursor

22

2.44

2.39

 gi|162461907

Stress-70 protein, mitochondrial

52

2.05

2.04

 gi|126521835

T-complex protein 1 subunit beta

18

1.71

1.68

 gi|6671702

T-complex protein 1 subunit epsilon

16

1.59

1.51

 gi|6753324

T-complex protein 1 subunit zeta

11

1.66

1.75

Protein transport

 gi|28077049

Charged multivesicular body protein 4b

9

0.51

0.51

 gi|51491845

Clathrin heavy chain 1

18

0.52

0.48

 gi|122939198

Clathrin light chain A isoform c

7

0.44

0.45

 gi|88014720

Importin subunit beta-1

9

0.41

0.42

 gi|124486712

Ribosome-binding protein 1 isoform a

20

0.60

0.65

Regulation of apoptosis and cell death

 gi|226874906

14-3-3 protein epsilon

13

2.01

1.62

 gi|6756041

14-3-3 protein zeta/delta isoform 1

15

2.55

2.61

 gi|6678682

Galectin-1

31

0.70

0.59

 gi|329755243

Gelsolin isoform 2

22

1.85

1.89

 gi|165932375

Plasminogen activator-inhibitor 1 RNA-binding protein

31

0.67

0.68

 gi|9790259

Programmed cell death protein 5

8

0.49

0.35

 gi|6755963

Voltage-dependent anion-selective channel protein 1

15

2.53

2.41

Regulation of canonical Wnt signalling

 gi|6679641

Emerin

8

2.87

3.42

 gi|7305075

ras GTPase-activating protein-binding protein 1

16

2.25

1.99

 gi|158854016

S-phase kinase-associated protein 1

7

0.50

0.50

Regulation of cell proliferation

 gi|124517663

Annexin A1

28

2.44

1.88

 gi|110625813

Astrocyte-derived neurotrophic factor precursor

11

0.47

0.47

 gi|28461294

Protein CDV3 isoform b

8

0.52

0.57

 gi|356640163

Serine hydroxymethyltransferase, mitochondrial

17

1.90

1.72

 gi|6678483

Ubiquitin-like modifier-activating enzyme 1

15

0.59

0.58

Transcription

 gi|188497724

Hepatoma-derived growth factor

12

0.55

0.50

 gi|84875537

Nucleolin

33

3.60

4.17

 gi|6679567

Polymerase I and transcript release factor

17

2.23

2.65

 gi|13386026

UPF0568 protein C14orf166 homolog

9

0.60

0.60

Translation and ribosome assembly

 gi|309266241

60S ribosomal protein L29-like

4

1.90

1.90

 gi|309263511

60S ribosomal protein L32-like

6

7.27

9.17

 gi|21426889

40S ribosomal protein S11

11

2.99

2.84

 gi|13386034

40S ribosomal protein S13

10

4.74

6.81

 gi|6677799

40S ribosomal protein S15

11

2.26

1.82

 gi|12963511

40S ribosomal protein S19

10

2.92

3.02

 gi|18087805

40S ribosomal protein S2

14

2.75

2.88

 gi|13195604

40S ribosomal protein S23

7

5.35

4.83

 gi|6755372

40S ribosomal protein S3

16

1.64

1.65

 gi|254553321

40S ribosomal protein S3a

13

2.75

2.65

 gi|6677805

40S ribosomal protein S4, X isoform

14

7.60

4.25

 gi|6677813

40S ribosomal protein S8

12

5.30

5.39

 gi|33504483

40S ribosomal protein S9

11

4.41

4.01

 gi|6671569

60S acidic ribosomal protein P0

10

1.40

1.56

 gi|83745120

60S acidic ribosomal protein P2

14

0.31

0.30

 gi|16418339

60S ribosomal protein L10

11

3.04

3.81

 gi|31981945

60S ribosomal protein L13a

9

3.96

3.00

 gi|13385036

60S ribosomal protein L15

7

4.40

3.52

 gi|83699424

60S ribosomal protein L18

5

3.03

3.22

 gi|58037465

60S ribosomal protein L18a

6

2.99

2.92

 gi|226958657

60S ribosomal protein L19 isoform 2

7

4.35

4.19

 gi|18250296

60S ribosomal protein L24

6

4.45

4.11

 gi|6677777

60S ribosomal protein L26

7

3.80

3.31

 gi|8567400

60S ribosomal protein L27

9

5.65

5.99

 gi|255308899

60S ribosomal protein L3

20

3.47

3.23

 gi|94386224

60S ribosomal protein L36-like

4

9.03

8.15

 gi|30794450

60S ribosomal protein L4

20

5.75

5.20

 gi|23956082

60S ribosomal protein L5

10

2.04

1.32

 gi|84662736

60S ribosomal protein L6

11

7.24

8.24

 gi|31981515

60S ribosomal protein L7

9

5.65

6.23

 gi|7305443

60S ribosomal protein L7a

9

6.30

6.42

 gi|6755358

60S ribosomal protein L8

7

8.87

6.51

 gi|14149647

60S ribosomal protein L9

9

1.81

2.29

 gi|254540168

78 kDa glucose-regulated protein precursor

49

5.20

4.12

 gi|82617575

Bifunctional glutamate/proline--tRNA ligase

6

0.43

0.44

 gi|126032329

Elongation factor 1-alpha 1

33

2.48

2.63

 gi|31980922

Elongation factor 1-beta

16

0.39

0.30

 gi|33859482

Elongation factor 2

44

0.32

0.50

 gi|21450625

Eukaryotic initiation factor 4A-I isoform 1

23

2.29

1.96

 gi|146219837

Eukaryotic translation initiation factor 3

19

0.50

0.63

 gi|365906249

Eukaryotic translation initiation factor 3

8

0.49

0.32

 gi|167234372

Eukaryotic translation initiation factor 4B

23

0.59

0.60

 gi|31712036

Eukaryotic translation initiation factor 5A-1

26

0.53

0.54

 gi|94367038

40S ribosomal protein S6-like isoform 2

6

5.49

5.50

 gi|309264022

40S ribosomal protein SA-like

16

3.02

4.32

 gi|149251177

60S ribosomal protein L13-like

8

4.11

3.18

 gi|377837258

60S ribosomal protein L23-like

8

3.83

3.84

 gi|63572172

60S ribosomal protein L27a-like

4

6.99

5.21

 gi|37497112

Putative RNA-binding protein 3 isoform 1

8

0.59

0.55

 gi|31982373

rRNA 2′-O-methyltransferase fibrillarin

8

2.15

2.07

  1. iTRAQ isobaric tags for relative quantification, PG progerin