Fig. 7

Histological analysis around the Ti implant in vivo. In vivo bone morphogenesis around Ti implants as observed under × 100 magnification at 4 weeks after implantation (a–d) and 8 weeks after implantation (e–h). (a, e) N-PBS implants, (b, f) P-PBS implants, (c, g) N-CM implants, (d, h) P-CM implants. Bar indicates 100 μm. Average histomorphometric values of bone implant contact (BIC) (i) and bone area fraction occupancy (BAFO) (j). Data are presented as mean ± SD (n = 3) for panels (i) and (j). *P < 0.05, **P < 0.01. N-CM plasma-untreated Ti on which SHED-CM was immobilized, N-PBS plasma-untreated Ti on which PBS was immobilized, P-CM Plasma-treated Ti on which SHED-CM was immobilized, P-PBS Plasma-treated Ti on which PBS was immobilized