Fig. 6

Immunohistological analysis at 5 weeks after transplantation of diPSC-NPCs into spinal cord-injured mice. a, b Images of horizontal sections of the spinal cord were taken. c The percentage of each neural cell type derived from transplanted diPSC-NPCs among total human nuclei⁺ cells is indicated in the graph. d, e Double immunostaining was performed with antibodies recognizing human nuclei and Tuj1. f A horizontal section of the spinal cord was immunostained with antibodies for human nuclei and MAP2. g Double immunostaining of a horizontal section of the spinal cord was performed with antibodies for human nuclei and Nestin. h Z-axis scanning of human nuclei and Nestin double-positive cells. i Double immunostaining with antibodies specific for human nuclei and GFAP was performed. j Double immunostaining with antibodies specific for human nuclei and NG2 was performed. k An example of human nuclei⁺ cell that was co-stained with Olig2 is shown. Data are presented as the mean ± standard error of the mean. diPSC disc cell-derived induced pluripotent stem cell, GFAP glial fibrillary-associated protein, MAP2 microtubule-associated protein 2, NG2 neural/glial anigen-2, NPC neural precursor cell, Olig2 oligodendrocyte lineage transcription factor 2, TUJ1 beta-III tubulin