Fig. 4

Sensitized regulatory T cells induce suppression of inflammatory responses in the liver. a Splenomagaly in HAT-A (1) mice in comparison with HAT-AT (2) and HA (3) mice. b Gross macroscopic abnormalities in mice. Mice with anomalies in various organs were counted, and percentage of mice with each anomaly was calculated. Most of the HAT-A mice showed abnormalities with respect to two to three indicators, whereas more than 90 % of the HAT-AT mice were normal. c Inflammation in the liver by histopathological analysis of HAT-A and HAT-AT mice after 120 days of transplantation. Representative images are shown for scoring of various inflammatory parameters: (1) portal inflammation, (2) periportal necro-inflammation, (3) endotheliasis, (4) necrosis, (5) sinusoidal lymphocyte infiltration, and (6) lobular necro-inflammation. Arrow indicated different immunoreactions with respect to the said parameters (scale of 100 μm; 200× magnification). d Comparative inflammatory scoring on the basis of histopathological analysis of the liver at different time intervals after transplantation in HAT-A and HAT-AT groups (*P < 0.05, **P < 0.01). HAT-A hemophilia A mice transplanted with allogeneic cells, HAT-AT hemophilia A mice transplanted with allogeneic and regulatory T cells, n number of mice in each time point