Fig. 7

Engraftment and regeneration of liver by donor-derived hepatocytes. a Representative photomicrographs show immunohistochemistry analysis of liver sections of HAT-A and HAT-AT mice after 4 months of transplantation. Sections were stained with anti-GFP/donkey anti-mouse Alexafluor 488 (scale of 100 μm; 200× magnification). Statistical analysis of GFP⁺ cells (indicated by white arrow) is shown in the right panel. b Immunohistochemical analysis of HAT-AT mouse liver sections after 4 months of transplantation. Representative images (scale of 20 μm; 630× magnification) show donor derived hepatocytes (anti-GFP/donkey anti-mouse Alexa fluor 488 and anti-albumin/donkey anti-goat Alexa fluor 594). c Quantitative analysis of GFP⁺ and GFP⁺albumin⁺ cells in HAT-AT liver (200× magnification) after 45 and 120 days of transplantation (*P = 0.03). d Representative images for Ki67⁺ donor cells in HAT-AT liver after 45 and 120 days of transplantation (scale of 10, 20 μm; 630 × 2.8 magnification). e Analysis of serum AST levels in HAT-AT and HAT-A mice after 45 and 90 days of transplantation. f Analysis of serum ALT levels in HAT-AT and HAT-A mice after 45 and 90 days of transplantation. ALB albumin, ALT alanine aminotransferase, AST aspartate aminotransferase, GFP green fluorescent protein, HAT-A hemophilia A mice transplanted with allogeneic cells, HAT-AT hemophilia A mice transplanted with allogeneic and regulatory T cells, IU international units, n number of mice in each experiment (***) P < 0.0001, (*) P < 0.05