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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Biological behavior of mesenchymal stem cells on poly-ε-caprolactone filaments and a strategy for tissue engineering of segments of the peripheral nerves

Fig. 2

Cell adhesion and viability in the presence of poly-caprolactone (PCL) filaments. a, b Confocal microscopy optical sections of mesenchymal stem cells (MSC) incubated at medium density (2 × 106 cells/mL) for 48 h on PCL filaments immunolabeled for CD90. b Orthogonal projections of Z-stack in high magnification, showing an individual PCL filament covered by two MCS. c Three-dimensional reconstruction by confocal microscopy of a single MSC (surface view) on an isolated PCL filament. d Quantitative analysis of the number of CD-90+ cells in contact with the PCL filaments and e the percentage of MSC aligned with the PCL filaments. f Percentage of viable cells in the different experimental conditions. Scale bars: a = 100 μm; b, c = 50 μm. ***p < 0.0001 by ANOVA. SC Schwann cells

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