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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Ex vivo immunosuppressive effects of mesenchymal stem cells on Crohn’s disease mucosal T cells are largely dependent on indoleamine 2,3-dioxygenase activity and cell-cell contact

Fig. 2

T-cell immunophenotype. The analysis of the expression of surface markers (CD3, CD4, CD8, CD25, and CD69) by T-cell lines generated in the absence (ctr-TCLs) and in the presence of mesenchymal stem cells (MSCs) at two different MSC-to-T cell ratios is shown. All markers were analysed in the gate of lymphocytes as delimited by side scatter-forward scatter parameters, but CD25 in that of CD4+ cells. The white panels indicate the use of T cells from healthy mucosa, the light grey panels indicate the use of T cells from non-inflamed mucosa, and the dark grey panels indicate the use of T cells from inflamed mucosa of patients with Crohn’s disease. For comments, see the text. Results are given as median (midline), 25th-75th percentiles (box), and extremes (whiskers) of at least three different T-cell lines for each condition. P values are as in Fig. 1. ctr-TCL control T-cell line, MSC-T T-cell lines obtained in the presence of mesenchymal stem cells

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Stem Cell Research & Therapy

ISSN: 1757-6512