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Table 2 Cytokine and HLA-G levels on cell-free supernatants with 1MT

From: Ex vivo immunosuppressive effects of mesenchymal stem cells on Crohn’s disease mucosal T cells are largely dependent on indoleamine 2,3-dioxygenase activity and cell-cell contact

 

Condition

TNF-α

IFN-γ

IL-6

IL-8

IL-10

IL-17A

IL-21

TGF-β1

HLA-G

(pg/ml)

(pg/ml)

(pg/ml)

(pg/ml)

(pg/ml)

(pg/ml)

(pg/ml)

(pg/ml)

(ng/ml)

Healthy mucosa

ctr-TCLs

55.3 ± 20.1

478.9 ± 100.7

409.8 ± 100.3

2005.4 ± 380.7

9.8 ± 2.8

18.9 ± 4.5

45.9 ± 9.5

555.9 ± 74.4

0.2 ± 0.07

 

n.s.

p < 0.05

p < 0.05

p < 0.01

p < 0.01

p < 0.001

p < 0.05

n.s.

n.s.

MSC:T 1:20

70.3 ± 17.9

998.4 ± 209.4

4690.5 ± 220.4

2499.7 ± 277.6

20.8 ± 5.7

18.6 ± 3.5

38.6 ± 9.8

499.2 ± 29.1

1.2 ± 0.4

 

n.s.

p < 0.01

n.s.

p < 0.01

p < 0.01

n.s.

p < 0.05

p < 0.05

n.s.

Non inflamed Crohn’s mucosa

ctr-TCLs

290.50 ± 108.4

654.65 ± 88.1

725.73 ± 117.9

2387.0 ± 328.4

11.5 ± 1.8

23.2 ± 2.9

57.5 ± 8.0

517.1 ± 26.9

1.2 ± 0.1

 

p < 0.05

p < 0.01

p < 0.05

p < 0.001

p < 0.001

p < 0.001

p < 0.01

p < 0.05

n.s.

MSC:T 1:20

402.3 ± 109.7

1117.8 ± 156.5

5154.68 ± 855.2

2408.1 ± 389.7

23.8 ± 3.8

23.6 ± 2.7

45.2 ± 4.7

567.4 ± 128.1

1.4 ± 0.3

 

n.s.

p < 0.05

n.s.

p < 0.001

p < 0.01

p < 0.05

p < 0.01

p < 0.05

p < 0.01

Inflamed Crohn’s mucosa

ctr-TCLs

428.2 ± 175.8

998.4 ± 328.4

3331.1 ± 538.2

2623.3 ± 475.8

14.1 ± 3.3

36.5 ± 4.2

128.9 ± 9.3

853.2 ± 97.0

1.1 ± 0.3

 

p < 0.01

p < 0.01

p < 0.01

p < 0.001

p < 0.001

p < 0.001

p < 0.001

p < 0.05

n.s.

MSC:T 1:20

827.4 ± 202.1

1290.3 ± 290.5

5468.4 ± 990.3

2318.9 ± 392.1

33.4 ± 7.3

13.2 ± 2.9

127.9 ± 8.3

1071.1 ± 197.7

1.3 ± 0.1

 

n.s.

p < 0.05

p < 0.01

p < 0.001

p < 0.01

p < 0.01

p < 0.05

p < 0.05

p < 0.01

  1. Data are expressed as mean ± standard deviation. The P values shown refer to the differences between the levels of each molecule on the supernatants of both the ctr-TCLs and MSC-TCLs at MSC-T cell 1:20 ratio in presence of 1-MT with those found in the cultures performed in the absence of 1-MT
  2. HLA human leukocyte antigen, TNF tumor necrosis factor, IFN interferon, IL interleukin, TGF transforming growth factor, ctr-TCL control T-cell line, n.s. not significant, MSC mesenchymal stem cell, 1-MT 1-methyl-DL-tryptophan