Fig. 1

Distinct morphological changes in hMSCs co-cultured with cancer cell lines. a–l GFP-labeled hMSCs were co-cultured with the indicated tumor cell lines at a 1:2 ratio, and on day 7 of the co-culture images were taken using a Nikon® ECLIPSE Ti-U inverted fluorescence microscope (4×). Data are representative of at least three independent experiments. m hMSCs co-cultured with normal breast epithelial cell line (MCF10A). n hMSCs (GFP-labeled) co-cultured with MCF7 (mcherry-labeled) as above. Cells were stained with nuclear staining DAPI (blue). o AT-MSCs labeled with DiO (green) and co-cultured with MCF7 cells labeled with DiD (red). At day 7, photomicrographs were taken using a Nikon® ECLIPSE Ti-U inverted fluorescence microscope (magnification 4×). p MCF7 cells were co-cultured with hMSCs at the indicated ratios (tumor:MSCs), and on day 7 images were captured in the green channel (MSCs) using 4× magnification. DAPI was used to stain nuclei