Skip to main content
Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: CDH1 and IL1-beta expression dictates FAK and MAPKK-dependent cross-talk between cancer cells and human mesenchymal stem cells

Fig. 3

Gene expression analyses of hMSCs sorted following direct contact with either MCF7 or BT-20 cells in co-culture systems revealed enrichment for genes related to bone formation. hMSCs were co-cultured with MCF7 or with BT-20 tumor for 7 days. The cells were trypsinized and hMSCs were sorted using magnetic activated cell sorting technology: a efficiency of sorting. b Hierarchical clustering of control hMSCs (blue) or hMSCs sorted from MCF7 (brown) or BT-20 (orange) co-cultures. Each column represents one replica. Expression level of each gene in a single sample is depicted according to the color scale. c Pie chart illustrating the distribution of the top 10 GO categories for the upregulated genes in sorted hMSCs from both MCF7 and BT-20 co-cultures. The pie section size corresponds to fold enrichment. d qRT-PCR validation of selected genes from the microarray data. Data are presented as mean ± standard error, n = 6. **P <0.005; ***P <0.0005. e ALP staining for control hMSCs (left) or hMSCs following co-culture with MCF7 (day 7) (middle). Representative photomicrograph of hMSCs (GFP-labeled) in co-culture with MCF7 (right). ALP alkaline phosphatase, GFP green fluorescent protein, hMSC human bone marrow-derived mesenchymal (stromal) stem cell, GO gene ontology

Back to article page