Fig. 5

High expression of CDH1 and low expression of IL-1β by tumor cells are associated with induction of hMSC morphological changes. a Clustering analysis performed on basal gene expression of the employed cancer cell lines indicated close clustering for the cancer cell lines that induce morphological changes in hMSCs (positive cell lines, blue; MCF7, BT-474, HT-29, and BT-20) relative to cancer cell lines that do not induce morphological changes in hMSCs (negative cell lines, red). b gene expression data for CDH1 and IL-1β in cancer cell lines retrieved from microarray data. c Complete abrogation of hMSC morphological changes when co-cultured with MCF7 (upper panels) or HT-29 (lower panels) in the presence of increasing dose of IL-1β. d Dose-dependent reversal into hMSC niche formation when co-cultured with FaDu cells alone or in the presence of the indicated concentrations of soluble recombinant IL-1 receptor (srIL-1R). Images were captured on day 7 and DAPI was used to stain nuclei. e Lack of hMSC niche formation when co-cultured with COLO-320 cells (CDH1 negative/IL-1β negative) compared with the HT-29 cells (CDH1 positive/IL-1β negative). CDH1 epithelial cadherin type 1, IL interleukin