Fig. 3
From: Development of a novel imaging system for cell therapy in the brain
NIS-Hipp-NSCs are viable, proliferate, and express NSC markers. MTS-based analysis of cell viability (a) and proliferation (b) of Hipp-NSCs transduced with the hNIS (NIS-Hipp-NSCs) compared with naïve Hipp-NSCs. N = 3; P > 0.05 by Student’s t test. c Hipp-NSCs and NIS-Hipp-NSCs were exposed to 99mTc for 30 min. MTS-based analysis of cell viability was performed 48 h later. N = 3; P > 0.05 by Student’s t test. d Western blot analysis of total protein extracts from NIS-Hipp-NSCs and naïve Hipp-NSCs grown under proliferating conditions. e Densitometric analysis. The expression of GAPDH was used to normalize the densitometry value index (DVI). N = 3; P > 0.05 by Student’s t test. GAPDH glyceraldehyde 3-phosphate dehydrogenase, Hipp-NSC hippocampus-derived neural stem cell, hNIS human sodium iodide symporter, MTS 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt, NIS-Hipp-NSC hippocampus-derived neural stem cell expressing the human sodium iodide symporter, NSC neural stem cell, 99m Tc technetium-99m