Fig. 5
From: Development of a novel imaging system for cell therapy in the brain
SPECT imaging of NIS-Hipp-NSCs. a SPECT signal from hNIS-Hipp-NSCs and Hipp-NSCs that were incubated with 99mTc for 30 min and washed in phosphate-buffered saline before imaging. The SPECT signal is shown superimposed on the CT scan image. b SPECT signal recorded from NIS-Hipp-NSCs that were incubated with 99mTc in vitro before transplantation in the right lateral ventricle. Transverse, coronal, and sagittal orientations of the brain are shown. c Analysis of the brain shows grafted cells (identified by the expression of GFP) in the ventricle. The expression of the NIS in the grafted cells was confirmed by immunofluorescence by using an antibody against hNIS (inset). CT computed tomography, GFP green fluorescent protein, Hipp-NSC hippocampus-derived neural stem cell, hNIS human sodium iodide symporter, NIS sodium iodide symporter, NIS-Hipp-NSC hippocampus-derived neural stem cell expressing the human sodium iodide symporter, SPECT single-photon emission tomography, 99m Tc technetium-99m