Fig. 8
From: Development of a novel imaging system for cell therapy in the brain
In vivo SPECT imaging of NIS-Hipp-NSCs injected in the brain hippocampus. Representative cross-sections of rat brain 2 weeks after transplantation of NIS-Hipp-NSCs or Hipp-NSCs in the hippocampus (CA1 region). Grafted cells are located on the basis of the expression of the cell tracker dye CMFDA. Mature neurons are identified on the basis of the expression of NeuN. Merged images show that some of the grafted cells express the neuronal marker NeuN (arrows). Cell nuclei are stained blue with DAPI. Scale bar = 50 μm. DAPI 4′,6-diamidino-2-phenylindole, Hipp-NSC hippocampus-derived neural stem cell, NIS-Hipp-NSC hippocampus-derived neural stem cell expressing the human sodium iodide symporter, SPECT single-photon emission tomography