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Table 1 List of antibodies used for the phenotypical characterization of Hipp-NSCs and hNIS-Hipp-NSCs

From: Development of a novel imaging system for cell therapy in the brain

Primary antibody

Specificity

Dilution

Source

Mouse anti-nestin

Neural progenitor cells

1:5000 WB

Millipore

1:200 IF

Rabbit anti-SOX2

Neural progenitor cells

1:2000 WB

Abcam

1:100 IF

Mouse anti-βIII-tubulin

Immature and mature neurons

1:5000 WB

Promega

1:1000 IF

Mouse anti-NeuN

Mature neurons

1:2000 WB

Millipore

1:1000 IF

Rabbit anti-GFAP

Astrocytes

1:5000 WB

Dako

1:1000 IF

Mouse anti-oligodendrocyte specific protein

Oligodendrocytes

1:200,000 IF

Millipore

Mouse anti-hNIS

Human NIS

1:100 IF

Abcam

  1. Hipp-NSC hippocampus-derived neural stem cell, hNIS-Hipp-NSC hippocampus-derived neural stem cell expressing the human sodium iodide symporter, WB Western blot, IF immunofluorescence, anti-GFAP anti-glial fibrillary acidic protein