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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Cis-existence of H3K27me3 and H3K36me2 in mouse embryonic stem cells revealed by specific ions of isobaric modification chromatogram

Fig. 4

Quantification of isobaric H3K27-R40me5 in mESCs and differentiated cells. a SIMC quantification of K27me3-K36me2 (green) and K27me2-K36me3 (red) isoforms of H3K27-R40me5 in histones from mESCs (D0) and at differentiation day 2 (D2) and day 4 (D4) induced by LIF withdrawal. b Relative abundance of each isoform of H3K27-R40me5 in mESCs and differentiated cells normalized to the H3D123-R128 peptide. The levels of H3K27-R40me5 and H3D123-R128 peptides were determined by SIC, and the amount of K27me2-K36me3 and K27me3-K36me2 isoforms was calculated by multiplying the total level of the H3K27-R40me5 by SIC and the constituent percentage of each isoform by SIMC. The MS experiment was repeated three times and the error bars represent the SD. ** P <0.01

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Stem Cell Research & Therapy

ISSN: 1757-6512