Fig. 3
let-7b upregulates survival signaling but downregulates autophagy-related molecules. a Intracellular caspase-3 (green) and let-7b (red) were visualized under confocal microscopy. The cells were either untreated (control), H2O2-treated (H2O2), or transfected with let-7b prior to H2O2 treatment (H2O2 + let-7b). Nuclei stained blue. Scale bar = 500 μm. b Various survival-related proteins were detected by western blotting. (*p <0.05, #p <0.05). c Caspase-3 activity and d cell survival was evaluated. (*p <0.05, #p <0.05 ). e Annexin V (green)/PI (red) staining. f TUNEL staining. g LC3A/B (green) was detected by confocal microscopy. Nuclei stained blue. Scale bar = 100 μm. h Expression of LC3A/B was detected by western blotting (*p <0.05, #p <0.05). i Expression of autophagy-related genes was evaluated by real-time PCR (**p <0.001, *p <0.05, #p <0.05). Quantitative data expressed as the mean ± standard deviation of at least three independent experiments