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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Early severe impairment of hematopoietic stem and progenitor cells from the bone marrow caused by CLP sepsis and endotoxemia in a humanized mice model

Fig. 1

Development of human hematopoietic cells in the bone marrow of NSG mice 8 weeks after transplantation of human CD34+ cells. Representative dot plots show flow cytometry analysis of bone marrow cells with specific anti-human monoclonal antibodies. a Diagram showing mean frequency of human leukocyte subsets in the bone marrow of humanized mice. b Staining of bone marrow from non-humanized NSG mouse with anti-human CD45. c Morphology of bone marrow cells from hu-NSG. d Expression of pan-hematopoietic CD45 antigen in cells gated in R1. e Analysis of monocytes. f Analysis of B cells. g Analysis of T cells. h Analysis of myeloid cells. i Analysis of hematopoietic stem and progenitor cells (gated from R1). j Histogram comparing expression of TLR4 receptor on HSCs and progenitor cells (gated from i). k Expression of TLR4 is present on both undifferentiated Lineage− cells and mature Lineage+ cells. The values on graphs present percentages of a given population from a maternal gate. HSC hematopoietic stem cell, hu-NSG humanized NOD.Cg-Prkdc/scidIL2rγ, NSG NOD.Cg-Prkdc/scidIL2rγ, TLR Toll-like receptor

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Stem Cell Research & Therapy

ISSN: 1757-6512