Fig. 5

Impact of LPS on the differentiation and proliferation of purified CD34⁺ human BM cells in vitro. a Effect of LPS on the frequency of the CD34⁺ CD38⁻ subpopulation after 9 days of culture. b Effect of LPS on the proliferative index of CD34⁺ CD38⁺ subpopulation. c Proliferation of CD34⁺ CD38⁺ cells in the presence of LPS. Impact of LPS on the frequency of CD34⁺ CD38⁻ cells (d) and CD34⁺ CD38⁺ cells (e) within first, second, and third generations of daughter cells (fewer than four generations). All results obtained after 9 days of culture in atmosphere of 1 % O₂. n = 6, *P < 0.05, **P < 0.001. BM bone marrow, LPS lipopolysaccharide